2025 Annual Report

“A particular pattern of mycobiome development may be necessary to impart resistance to the development of BPD.”

KENT WILLIS, M.D

64 2.5 lbs 1.6 lbs 38 very-preterm infants who did not develop BPD had an average birthweight of very-preterm infants who did develop BPD had an average birthweight of

with an antifungal agent before birth, that inhibition of perinatal fungal colonization reduced lung injury in the newborn pups. In contrast, a gain-of-function experiment, where the perinatal fungal colonization of dams was augmented with a species of Candida fungus common in mice, amplified BPD severity in the newborn pups. “These findings demonstrate that features of the initial intestinal fungal micro biome are associated with the later development of BPD in premature neonates and exert a microbiome-driven effect that is transferable and modifiable in mouse models,” said Kent Willis, M.D., assistant professor in the UAB Department of Pedi atrics Division of Neonatology. “This suggests causality, and it suggests that the gut fungi may represent a therapeutic target in newborn lung disease.” Willis and Ajay J. Talati, M.D., University of Tennessee Health Science Center, Memphis, Tennessee, co-led the study , published in Microbiome. “Collectively, our analyses demonstrate that the composition of the intestinal mycobiome of infants who did not develop BPD was more uniform,” Willis said. “In contrast, those who eventually developed BPD had more disparate mycobiomes. This suggests that a particular pattern of mycobiome development may be necessary to impart resistance to the development of BPD, and failure to do so in various ways is associated with disease development.” The first defecations of newborn infants are meconium, composed from materials ingested while in the uterus. The first true stools in the second week, the ones analyzed by Willis and Talati, are digested milk. It is known, Willis and Talati say, that fungi in adults are vital members of the human microbiota; but compared to bacteria, their non-pathological and non-parasitic functions are still poorly understood, especially in newborns. This prospective observational cohort study included newborn stool samples collected over six years from 2017-2020 in Memphis and 2021-2022 in Birmingham. The 64 very-preterm infants in the study who did not develop BPD had an average birthweight of 2.5 pounds, and the 38 very-preterm infants who did develop BPD had an average birthweight of 1.6 pounds. Only one of the 64 no-BPD infants in the study died, while six of the 38 BPD infants died. ●

Co-authors with Willis and Talati in the study , “The fungal microbiota modulate neonatal oxygen-induced lung injury,” are Isaac Martin, Mary Silverberg, Ahmed Abdelgawad, Kosuke Tanaka, Brian A. Halloran, Teodora Nicola, Samuel J. Gentle, Namasivayam Ambalavanan, Viral G. Jain, Charitharth V. Lal and Tamás Jilling, UAB Department of Pediatrics Division of Neonatology; Erin D. Myers, Jay P. Desai, Catrina T. White and Brian M. Peters, University of Tennessee Health Science Center; Ibrahim Karabayir and Oguz Akbilgic, Wake Forest University, Winston-Salem, North Caroli na; Laura Tipton, James Madison University, Harrisonburg, Virginia; Luan D. Vu, University of Texas at San Antonio, San Antonio, Texas; Stephania A. Cormier, Louisiana State University, Baton Rouge, Louisiana; and Joseph F. Pierre, University of Wisconsin-Madison, Madison, Wisconsin.

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2025 ACADEMIC ANNUAL REPORT